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Briefly, a core cardiac biopsy was taken from the peri-infarct zone, fresh-frozen and sectioned in cryosection medium (see online supplementary material). Adjacent tissue sections were stained with HE for conventional, smooth muscle actin, CD31, CD68 (clone PG-M1) and integrin αvβ3 antibody clone LM609 (Millipore) before digital imaging (Axioscan.Z1, Zeiss, UK) and histopathological examination.

αvβ3 integrin expression in patient with recent myocardial infarction (MI). Adjacent fresh-frozen and cryosectioned biopsies from the peri-infarct area of a patient with recent anterior MI are shown. Immunohistochemical staining for (A) αvβ3 integrin displayed multiple regions of positive staining that co-localise to regions of staining for vascular endothelial cells (CD31), (B) visible at 4 magnification. At 20 magnification, these regions of αvβ3 staining (C) correspond predominantly to arterioles and the microvasculature (D) and also to regions of staining for smooth muscle actin (SMA) (E), representative of both arterioles (co-staining with CD31) and myofibroblasts. There were relatively few macrophages (F).


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